Hi @smal_creative
Yes, I have done this many times with no issues.
I think your issue is related to the JS side of things. Please note that ACF is a PHP API for saving and loading data, not a library for making sliders and such.
Perhaps you need to create a sandbox to test running 2 sliders on the same page, then when it is working, you can populate the slides with ACF data.
Good luck
Thanks
E