Probably, unless it is a sub field.
Generally, it’s not a good idea to insert values into the DB without using ACF update_field() using the field key as I mentioned above, or not inserting the field key reference if you’re using some other way to insert the data. Because of the various ways that fields can be added there isn’t any way to predict what might not work. The only way that you can be sure that things will work is if you edit and update every post in the admin after the data is added which likely defeats the purpose of adding it any other way. All import plugins that are ACF aware manage this.
